Figure 2
From: Immune-responsiveness of CD4+ T cells during Streptococcus suis serotype 2 infection
Total splenocytes produce ex vivo type-1 pro-inflammatory cytokines in response to S. suis systemic infection.
Mice were infected intraperitoneally with a dose of 5 × 107 CFU of S. suis wild-type strain P1/7 (n = 3 per group × 3 individual experimental infections). Spleens were harvested 6 h post-infection and total splenocytes plated at 5 × 106 cells/well. After 6 h of incubation, gentamycin was added to the culture to prevent cell toxicity. Cells were then incubated for 48 h and supernatants were collected for cytokine analysis by ELISA. Non-stimulated cells from mock-infected animals served as negative control for basal expression (C−). Cells stimulated with Concanavalin A (0.1 μg/ml) were used as positive control (C+). Data are expressed as mean ± SEM (in pg/ml) from 3 different experimental infections. *P < 0.05 denotes values that are significantly higher than those obtained with splenocytes from non-infected mice (C−).
