Figure 1 | Scientific Reports

Figure 1

From: Analysis of chikungunya virus proteins reveals that non-structural proteins nsP2 and nsP3 exhibit RNA interference (RNAi) suppressor activity

Figure 1

Screening of CHIKV genes for RNAi suppressor activity.

(a) Schematic representation of CHIKV genome, showing non-structural and structural genes. (b) GFP reversion assay of all CHIKV genes in Sf21 sensor cell line. FHVB2 and NS4B were used as positive controls. The y-axis shows % GFP reversion (Mean ± SD). Statistical significance was analysed using student’s t-test and Fisher’s Least Significant Difference (LSD) test using empty vector as control. The *symbol indicates a statistically significant difference in terms of the P value (P < 0.001). nsP2, nsP3 and nsP4 were found statistically highly significant from LSD test (P value < 0.0001 each) when compared with the vector (negative control) (Alpha level of the test = 0.05). (c) Schematic representation of transfection methodology for Sf21 sensor cell line along with fluorescent microscopic images and FACS dot plot 72 h post transfection. Sf21 and Sf21 GFP stable cell lines were used as FACS gating control. All the transfections were performed in Sf21 sensor cell line having GFPshRNA stably integrated.

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