Figure 4 | Scientific Reports

Figure 4

From: Analysis of chikungunya virus proteins reveals that non-structural proteins nsP2 and nsP3 exhibit RNA interference (RNAi) suppressor activity

Figure 4

Reversion assay of GFP dsRNA and siRNA mediated silencing.

(a) Sf21 GFP stable cell line was silenced with 2.5 pmole GFP siRNA. Scrambled siRNA (sc-siRNA) was used as siRNA negative control. GFP reversion assay was performed by co-transfecting GFP siRNA and VSR plasmids. FACS analysis was done after 72 h. (b) Sf21-GFP stable cell line was silenced by transient transfection with GFPdsRNA plasmid. Reversion assay was performed by co-transfecting GFPdsRNA plasmid and VSR plasmid. FACS analysis was done after 72 h. From the FACS data percentage GFP reversion was calculated as ((percentage GFP upon co-transfection) − (percentage GFP after silencing)/(percentage GFP of GFP cell line) − (percentage GFP after silencing)) × 100.

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