Table 2 Primers used for cDNA amplification, dsRNA synthesis and qRT-PCR analysis.
Function | Primer | Sequence (5′–3′) |
|---|---|---|
cDNA amplification | Mi-ace1-F | ATGATGGATTATTCAATAGAGGACAG |
Mi-ace1-R | CTATTTTATTCCACAAACATCATTATCACC | |
Mi-ace2-F | GAGGTGAATTATGCGCAAACGAAG | |
Mi-ace2-R | TTATTTGAAAATAAATGATGAATACAGGAAAGATATTTCAGG | |
dsRNA template synthesis | T7-iace2-F | TAATACGACTCACTATAGGGGATGGTAAATCTGTTCACAC |
T7-iace2-R | TAATACGACTCACTATAGGGGCACGCTCTTGAACCG | |
T7-iegfp-F | TAATACGACTCACTATAGGGGAGTACAACTACAACAGCCAC | |
T7-iegfp-R | TAATACGACTCACTATAGGGACGAACTCCAGCAGGACCAT | |
qRT-PCR | q-actin-F | GGGTATGGAATCTGCTGGTAT |
q-actin-R | AGAAAGGACAGTGTTGGCGTA | |
q-ace2-F | GCTGGTGATGCTGTTTCTC | |
q-ace2-R | CCACAAGAATGCTAACATCACG |
