Figure 4: Increased ROS production in the BM cells and K562 cells treated with lactic acid in vitro.
From: The Promotion of Erythropoiesis via the Regulation of Reactive Oxygen Species by Lactic Acid
BM cells (a) and K562 cells (b) were treated with lactic acid for 6 hours and then pre-loaded with 10 μM DCFH-DA. DCF fluorescence was measured via flow cytometry. The black curves indicate lactic acid-treated K562 cells or BM cells, and the grey curves indicate untreated cells. MFI analyses are shown to the right. The data represent the mean ± S.E.M. (n = 3). *P < 0.05. (c) Enhanced production of H2O2 was observed in the K562 cells 15 minutes after the 10-mM lactic acid treatment. The data represent the mean ± S.E.M. *P < 0.05.
