Table 1 Chessboard titration of the optimal concentration of hapten and antibody.
From: A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles
The concentration of hapten (mg L−1) | The concentration of antibody (mg L−1) | |||||
|---|---|---|---|---|---|---|
3.02 | 1.51 | 0.76 | 0.38 | 0.30 | Negative control | |
3.21 | 17.75 ± 0.11 | 17.69 ± 0.13 | 18.06 ± 0.12 | 18.99 ± 0.13 | 19.52 ± 0.09 | 26.12 ± 0.07 |
1.60 | 17.65 ± 0.13 | 17.93 ± 0.08 | 18.31 ± 0.14 | 18.20 ± 0.12 | 19.48 ± 0.07 | 25.00 ± 0.10 |
0.80 | 17.74 ± 0.11 | 17.51 ± 0.09 | 18.49 ± 0.12 | 18.45 ± 0.13 | 19.71 ± 0.13 | 26.04 ± 0.08 |
0.64 | 17.99 ± 0.09 | 17.84 ± 0.10 | 18.29 ± 0.15 | 18.81 ± 0.10 | 19.97 ± 0.10 | 26.03 ± 0.06 |
0.43 | 18.26 ± 0.12 | 18.65 ± 0.14 | 18.22 ± 0.12 | 19.12 ± 0.13 | 19.31 ± 0.09 | 26.10 ± 0.09 |
