Figure 3
Alignment of amino acid sequences for the M1-M2 loop and M2 region in all of the nAChR subunits used in the present study.
Residues expected to line the nAChR pore (termed rings below) are depicted in bold and numbered 1–8 starting at the extracellular terminus. F in ring 3 of r-β4 (a difference that is conserved in other species including humans) is highlighted in orange, F and G in rings 5 and 6 (selectivity filter) of m-β1 are highlighted in green and the positively charged residues at the external mouth of the pore in m-β1, m-δ and m-γ are highlighted in blue.
