Figure 4
The effect of M2 mutations β2(V253F) and β4(F255V) on PhTX-343 and PhTX-12 inhibition.
Concentration-inhibition curves for PhTX-343 (A,B) and PhTX-12 (C,D) inhibition of α3β4 (blue ■), α3β4(F255V) (blue □), α4β2 (red ●) and α4β2(V253F) (red ○) peak (A,C) and late (B,D) current. The ACh concentrations were 10 μM for α4β2 and α4β2(V253F), and 100 μM for α3β4 and α3β4(F255V). VH = −80 mV. Curves are fitted by Eq. 1 and IC50 values are given in Tables 1 and 2.
