Figure 6
Recovery of responses to ACh following antagonism by PhTX-343 (A,B) and PhTX-12 (C,D) for peak current (A,C) and late current (B,D). A pre-toxin control response to ACh was obtained at −6 min, the response at time 0 was in the presence of PhTX, while the responses from 6 min and onwards were applications of ACh only. ACh was applied at 10 μM for α4β2 (green ●), α4β4 (blue ■) and α1β1γδ (orange 
), 30 μM for α3β2 (brown ▼) and 100 μM for α3β4 (red ▲) and α7 (purple ♦). PhTX-343 was applied at 10 μM for α4β2, α4β4, α3β4 and α3β2, 30 μM for α7 and 100 μM for α1β1γδ. PhTX-12 was applied at 10 μM for all subunit combinations. (E–G) Currents in response to ACh before PhTX application (black), ACh co-applied with PhTX (light grey), and ACh alone 6 min after co-application with PhTX (dark grey) for PhTX-343 inhibition of α3β4 (E) and α4β2 (F), or PhTX-12 inhibition of α4β2 (G). VH = −80 mV in all cases.
