Figure 3

Identification of glucansucrase Gsy in BD3749.
(A) The total proteins from the supernatant were prepared as described and equivalent amount of total protein was separated on a 16 × 20 cm 6% SDS-PAGE and stained with Coomassie brilliant blue G250. The lines on the right indicate the differentially expressed protein bands. (B) In situ detection of dextransucrase activity. After electrophoresis, the SDS-PAGE gel was incubated in 50 mM sodium acetate buffer containing 50 g/L sucrose buffered at pH 5.4. The active bands were detected by the appearance of opaque polymer.