Figure 4 | Scientific Reports

Figure 4

From: Gsy, a novel glucansucrase from Leuconostoc mesenteroides, mediates the formation of cell aggregates in response to oxidative stress

Figure 4

Expression analysis of glucansucrase-encoding genes.

(A) Equal amount of total protein from the supernant was separated by 6% SDS-PAGE and immunoblotted with the indicated antibodies after transfer onto PVDF membranes. The gel was run in triplicate and analysed with anti-1322, anti-1323, and anti-1322 plus anti-1323, respectively. (B) Effect of oxygen on the protein level of glucansucrases. Equal amount of total protein from cell pellets from oxic and anoxic cultures were separated and detected with the indicated antibody. (C) Transcription analysis of the putative glucansucrase genes in response to oxygen. The transcription level under oxic conditions was normalized to that under anoxic conditions. Asterisk indicates low transcription level that is close to the limit of detection.

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