Figure 7

Increase in EPS in response to oxidative stress.
(A) EPS synthesized by BD3749 under oxic and anoxic conditions. The insoluble and soluble EPS were collected and detected, respectively. (B) EPS synthesized by BD3749 in broth with H2O2 log phase culture of BD3749 was diluted 1:50 into fresh medium supplemented with H2O2 at the indicated concentration. After 48 h of cultivation, insoluble and soluble EPS in the culture were obtained as described in Materials and Methods. Excluding the oxic group, incubation was carried out in the Whitley A35 Anaerobic Workstation. The average amount of three experiments and standard deviations are shown.