Figure 5

Characterization of BAT.
(A) Representative hematoxylin and eosin sections; (B) Lipid droplet size and number (n = 10/group); (C) Expression of genes associated with thermogenesis, lipolysis, recruitment and β-adrenergic signaling (n = 7–10/group); (D) Quantification of BAT UCP1, pHSL and HSL protein by Western Blot analysis and normalization against β-actin as loading control (n = 4 mice/group). Data are presented as mean and SEM (*p < 0.05, **p < 0.01, ***p < 0.001).