Figure 1

SAMHD1 expression can be reliably examined using flow cytometric analysis.

(a) Dot-plots of SAMHD1 expression in THP-1 and Jurkat cell lines. THP-1 cells were treated with 4ng/mL SIV-Vpx for 24 h, or were untreated. SAMHD1-silent THP-1 cells (THP-1-shSAMHD1) or control shRNA (THP-1-shRNA Ctrl) were used as SAMHD1 staining controls. Percentages indicate percentage of SAMHD1⁺ cells, and digits represent MFI of SAMHD⁺ cells. (b) SAMHD1 expression was further confirmed using western blotting. Relative protein blot signal intensity analysis was performed using Li-Cor Image Studio software. Numbers represent relative value to the control or blank by normalization with to β-Actin. (c) PBMCs from an HIV-seronegative donor were stained with an antibody mixture with, or without, SAMHD1 pAb, a histogram illustrating SAMHD1 expression. (d) SAMHD1 positive and negative subgroups were sorted and expression of SAMHD1 was confirmed using western blotting. (e) Gating strategy results for SAMHD1 expression in activated CD4⁺ T cells, resting CD4⁺ T cells, and monocytes of PBMCs from an HIV-seronegative individual are presented. Percentages indicate percentage of SAMHD1⁺ cells.