Figure 2

SAMHD1 expression and Vpx-mediated SAMHD1 degradation in HIV-1 target cells.

(a) Representative flow cytometric analysis results for SAMHD1 expression in activated CD4⁺ T cells, resting CD4⁺ T cells, and monocytes from HC, cART-naive, and cART patients are presented. Numbers in top right indicate percent SAMHD1 positive cells. (b) Statistical analysis of data was obtained for n = 25 HC, n = 22 cART-naive individuals, or for n = 17 cART individuals. (c) Fresh isolated PBMCs were treated with 2 ng/mL SIV-Vpx or SIV-Mock for 48 h, and the expression of SAMHD1 in activated CD4⁺ T cells, resting CD4⁺ T cells, and monocytes from HC, cART-naive, and cART cohorts was determined. Representative histograms were shown. Numbers in top left indicate percent SAMHD1 positive cells in SIV-Mock (grey), and SIV-Vpx treatment (black), groups. (d) Shown are summary results for the effects of SIV-Vpx (“∆”) or SIV-Mock (“○”) on SAMHD1 expression in activated CD4⁺ T cells, resting CD4⁺ T cells, and monocytes from 10 HC, 22 cART-naive individuals, and 17 cART individuals. Numbers represent the mean value of loss of SAMHD1 positive cells after SIV-Vpx treatment. The significance was measured using the Mann-Whitney test, *p < 0.05, **p < 0.001, ***p < 0.0001; n.s, not significant.