Figure 3

Relationship of SAMHD1 expression and degradation with in vivo HIV-1 replication.

(a) Results of statistical analysis of the correlation between viral load and SAMHD1 expression in activated CD4⁺ T cells (“○”), resting CD4⁺ T cells (“▵”), or monocytes (“×”), in cART-naive patients (n = 22). Correlation statistics were analyzed using the Spearman correlation. r, Spearman’s correlation coefficient. (b) The proportions of SAMHD1 positive cells in activated CD4⁺ T cells, resting CD4⁺ T cells, and monocytes are presented for the chronic viremia (CHVIR, VL>10000 copies/mL), and viremic controllers (VC, VL<2000 copies/mL), cohorts, CHVIR, n = 8; VC, n = 6. Comparisons were made using the Mann-Whitney test, p-value is not indicated when p > 0.05. (c,d) There was no correlation for activated CD4⁺ T cells, whereas there was a negative correlation, between the frequency of cells susceptible to SAMHD1 degradation in monocytes and plasma viral loads from cART-naive subjects (n = 22). Correlation statistics were analyzed using the Spearman correlation. r, Spearman’s correlation coefficient. (e,f) The frequency of SAMHD1 degradation by SIV-Vpx in activated CD4⁺ T cells, and monocytes was compared between CHVIR and VC cohorts. Numbers represent the mean value of loss of SAMHD1 positive cells after SIV-Vpx treatment. p values were evaluated using the Mann-Whitney test, *p < 0.05, **p < 0.001, ***p < 0.0001; n.s, not significant. SIV-Vpx (“▵”), SIV-Mock (“○”).