Figure 2
Rapamycin treatment promotes a hepatic autophagy-like process targeting EEFs.
Hepa1-6 cells growing on a coverslip were incubated with P. y. yoelii 265BY-RFP sporozoites for 3 h. The cells were treated with the indicated stimulus for 24 h and then stained with anti-LC3 and anti-LAMP1, counterstained with DAPI, and subjected to confocal microscopy. (A) A representative confocal microscopy image of an autophagosome containing EEFs and the fusion of the autophagosome with a lysosome after incubation with the indicated compounds. (B) Statistical analysis of the autophagosomes containing EEFs. (C) Statistical analysis of the fusion of the autophagosomes containing EEFs with the lysosomes. One of three individual experiments is presented, and all data are presented as the mean ± SD. *P < 0.05; ** P < 0.01.
