Figure 5 | Scientific Reports

Figure 5

From: The rodent malaria liver stage survives in the rapamycin-induced autophagosome of infected Hepa1–6 cells

Figure 5

The maturation of EEF-containing autolysosomes was inhibited.

After Hep1-6 cells were incubated with P. y. yoelii 265BY-RFP for 48 h, the cells were stained with anti-LC3, anti-LAMP1 and anti-cathepsin D, then subjected to confocal microscopy. (A) A representative confocal image of EEF in cathepsin D-negative autolysosome. (B) Quantification of EEF in cathepsin D-negative autolysosomes. Three individual experiments were performed, and all data are presented as the mean ± SD.

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