Figure 5 | Scientific Reports

Figure 5

From: Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface

Figure 5

Effect of substitutions in CDRH3 on Fab binding to the 10E8 epitope in the context of the plama membrane and correlation with neutralizing activity.

(a) JRCSF PsV recognition by 10E8 Fab and mutein Fabs in a dot-blot assay. Decreasing amounts of PsV (from left to right) were spotted onto nitrocellulose membranes and probed with 10E8 Fab and its muteins. (b) Cell lysates bearing recombinant MPER proteins (MPER-TM1: left; MPER-PDGFR: right) were probed with 20 nM IgG or Fab, as indicated, and binding signals were normalized to that of 20 nM 17/9 IgG, which binds to N-terminal HA tags on both constructs. (c) Neutralization profile of a panel of PsVs bearing HIV Env or that of vesicular stomatitis virus (negative control). IC50 and IC90 values for 2F5, 4E10 and 10E8 IgGs, 10E8 Fab and its muteins are shown.

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