Table 1 Thermodynamic parameters of binding of Fab 10E8 to the peptide 10E8ep and peptides based on Helix 2.

From: Structural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface

Peptide

KD(nM)

ΔG° (kcal mol−1)

ΔH° (kcal mol−1)

−TΔS° (kcal mol−1)a

n b

10E8ep

9.6 ± 1.0

−10.9 ± 0.1

−9.6 ± 0.1

−1.3 ± 0.2

1.0 ± 0.1

H2 (683)

746 ± 307

−8.3 ± 0.3

−3.3 ± 0.3

−5.0 ± 0.6

0.9 ± 0.1

H2 (686)

369 ± 84

−8.7 ± 0.1

−9.6 ± 0.5

0.9 ± 0.6

0.9 ± 0.1

H2 (690)

10 ± 1.6

−10.9 ± 0.1

−8.4 ± 0.1

−2.5 ± 0.2

0.8 ± 0.1

H2 (693)

9.4 ± 2.0

−10.9 ± 0.1

−9.0 ± 0.1

−1.9 ± 0.2

1.3 ± 0.1

  1. aTemperature was 298 °K.
  2. bn refers to the molar ratio peptide/protein.
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