Figure 2 | Scientific Reports

Figure 2

From: Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows

Figure 2

Changes in the relative amounts of RIPK1 and RIPK3 mRNA and protein expression in the bovine CL tissues during PGF-induced luteolysis.

(A) and (B) Comparison of relative amounts of RIPK1 or RIPK3 mRNA determined by quantitative RT-PCR in bovine CL tissue after PGF administration (0 h: control; 2 h, 4 h and 12 h). Data are the mean ± SEM for four samples/stage and are expressed as the relative ratio of RIPK1 or RIPK3 mRNA to GAPDH mRNA. (C) and (D) Representative western blot bands for RIPK1 or RIPK3 and ACTB. Densitometrically analyzed western blot results in bovine CL tissue during different luteal phases. Data are the mean ± SEM for four samples/stage and are expressed as the relative ratio of RIPK1 or RIPK3 protein to ACTB protein. The data were statistically analyzed by a non-parametric Kruskal-Wallis test followed by Dunnett’s multiple comparison test. Different superscript letters indicate significant differences (P < 0.05).

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