Figure 2
From: MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii
Screening cell lines overexpressing Cre-miR906-3p or Cre-miR910.
(A) Strategy for the construction of miRNA-overexpressing vector, which includes PsaD promoter (PPsaD), luciferase gene, and Cre-miR906-3p/Cre-miR910 miRNA-overexpressing precursor; (B) Screening Cre-miR906-3p-overexpressing cell lines by determining luciferase activity; (C) Screening Cre-miR910-overexpressing cell lines by determining luciferase activity; (D) q-PCR validation of the expression level of Cre-miR906-3p in the cell lines; (E) q-PCR validation of the expression level of Cre-miR910 in the cell lines. The expression folds of miRNA and its target gene were measured in the same samples; *Indicates that differences between the overexpressing lines and the wild-type lines were significant at P < 0.05.
