Figure 5
From: MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii
Screening cell lines overexpressing ATP4 or NCR2 genes.
(A) miRNA-mRNA pairing: Cre-miR906-3p and ATP4 (up); Cre-miR910 and NCR2 (down); (B) Strategy for the construction of miRNA-overexpressing vector, which includes the HSP70-RBCS2 promoter, HA tail, and ATP4/NCR2 gene; (C) q-PCR validation of the expression level of ATP4 in the cell lines; (D) q-PCR validation of the expression level of NCR2 in the cell lines. The expression folds of miRNA and its target gene were measured in the same samples; *indicates that differences between the overexpressing lines and the wild-type lines were significant at P < 0.05.
