Figure 1
From: Defining an olfactory receptor function in airway smooth muscle cells
Expression of olfactory receptor mRNA transcripts in human ASM and transfected HEK-293T cells.
(a) Golf and AC3 are both expressed in human ASM cells (sequenced to confirm). (b) The five ORs transcripts identified by degenerate PCR were also confirmed in human ASM, using gene-specific primers to each OR. Mock RT samples (below) serve as negative controls. (c) Confirmed expression of the full-length transcript of OR51E2 in RT, but not in mRT. (d) Three of the five ASM ORs are able to traffic to the surface of HEK-293T cells. The top panel shows cell surface OR population as detected by immunostaining against an N-terminal extracellular FLAG epitope tag with a polyclonal antibody. For detecting the internal OR population, cells were subsequently fixed, permeabilized, and stained again for Flag using a monoclonal antibody (bottom panel). All constructs with the exception of OR51E2 contain a leucine rich N-terminal signal peptide and are co-transfected with chaperon proteins as previously described26,34; “+” and “−” indicate cell surface localization of ORs.
