Figure 2

Human body atlas of ASM OR.

(a) Expression of the five de novo identified ASM ORs across 30 human tissue types (GTEx RNA-Seq tissues). Each colored block provides a quantile spread representing expression levels expressed as fragments per kilobase of transcript per million mapped reads (FPKM) across all samples of that tissue type. The color at the middle of the strip represents the median expression, and the color at the end of the strip represents the maximum expression. A complete absence of reads over an OR is represented as grey. The number of human samples in the collection is displayed next to the tissue name. (b) Cultured lung cell expression of the indicated ORs (also see Supplementary Table 1). (c) RT-PCR cross-validation of the most abundant lung-resident OR genes (shown in panel b) in human ASM cells isolated from 6 non-asthmatic lung donors. Results are shown as 2^−ΔCt X 10000, where ΔCt = [Ct(target gene) − Ct(gapdh)].