Figure 4

Characteristics of the OR51E2 homologue Olfr78.

(a) Cytoskeletal dynamics of ASM cells derived from several inbred mouse strains as measured by SNTM. Data are presented as mean ± SE (AJ, n = 825; BALB/c, n = 599; C57BL/6, n = 805 individual beads measurements). (b) A schematic diagram of murine Olfr78 locus and its exons; three target sites in exon 4 are shown below, indicating the CRISPR N20NGG target sites. (c) Genome editing efficiency of CRISPR-Cas9 in the primary mouse ASM cells as determined by western blot. Full-length gels/blots are presented in Supplementary Figure 7a. (d) Computed MSD at 300 s for Olfr78 WT and Olfr78 KO cells (C57BL/6) in response to 24 h exposures to acetate and propionate (10 mM). Data are presented as geometric mean ± 95% CI (n = 307–379 individual beads measurements for each group). (e) Isolated mouse ASM cells (C57BL/6) were transfected with or without FLAG-tagged full-length construct encoding human OR51E2. OR51E2 protein expression was detected by anti-Flag antibody. Full-length gels/blots are presented in Supplementary Figure 7b. Although the expected band is ~36 kDa, ORs typically also appear as multiple higher molecular weight bands³⁴. (f) Computed MSD at 300 s for wild-type (WT) and OR51E2-overexpressing (Flag-OR51E2) mouse ASM cells. Data are presented as geometric mean ± 95% confidence interval (n = 354–364 individual beads measurements for each group).