Figure 5
From: Defining an olfactory receptor function in airway smooth muscle cells
Activation of OR51E2 inhibits ASM proliferation.
(a) Representative photomicrographs of cells treated for 24 h with or without formate, acetate, and propionate (10 mM) as measured by EdU cell proliferation assay. EdU positive nuclei are labeled with Alexa Fluor 647 (purple), and all nuclei are labeled with DAPI (blue). (b) The ratio of Alexa Fluor 647 positive and DAPI positive nuclei were counted in four non-overlapping fields per sample. Data are presented as mean ± SE (n = 3 independent samples). (c) ASM proliferation was measured by live cell counting over 6 days in culture with SCFAs. Data are presented as mean ± SE (n = 4 independent measurements). (d) The effects of SCFAs on cellular proliferation of WT and OR51E2 KO cells as quantified by Cell Counting Kit-8 (CCK-8, Sigma). For these studies, cells were treated for 24 h with 10 mM SCFAs and then 1 mM SCFAs for the next 5 days in culture. CCK-8 assay was performed on day 6. Data are presented as mean ± SE (n = 3 independent measurements). (e) The relative transcript levels of OR51E2 in isolated human ASM (non-asthmatics, n = 6 lung donors; asthmatics, n = 6 lung donors). (f) The effects of SCFAs, acetate and propionate, on cellular proliferation of asthmatic ASM derived from 6 independent lung donors as measured by live cell counting over 6 days in culture.
