Figure 5
Effects of SCH on NF-κB and IκB expression in LPS-stimulated and SCH-treated BV2 cells.
LPS-induced BV2 microglial cells were treated with different concentrations of SCH for 24 hr. (a) The levels of NF-κB and IκB were detected by Western Blotting. β-actin was used as an internal control. Each blot was representative of at least three independent experiments. **p < 0.001 versus control; ##p < 0.01 versus LPS (1 μ/ml) group. (b) The density of corresponding bands in (A) were quantitated and plotted as the ratio of NF-κB /β-actin. (c) The density of corresponding bands in (A) were quantitated and plotted as the ratio of IκB /β-actin. (d) Immunofluorescence result for p-NF-κB (green) in LPS-stimulated and SCH-treated BV2 cells. Scale bar = 200 μm. Magnification of 5x within the white rectangles in image below. p-NF-κB located in or around the nucleus by white arrows. Scale bar = 40 μm. Full-length blots are presented in Supplementary Fig. S1.
