Figure 6 | Scientific Reports

Figure 6

From: Interference with Protease-activated Receptor 1 Alleviates Neuronal Cell Death Induced by Lipopolysaccharide-Stimulated Microglial Cells through the PI3K/Akt Pathway

Figure 6

Effects of the conditioned medium from LPS-stimulated and SCH-treated BV2 microglia on PC12 cells

. (a) After treatment of the conditioned medium for 24 hr, cell viability was measured by the MTT assay. (b) Bax, Bcl-2 and β -actin was detected by We stern blotting analysis. (c) The density of corresponding bands in (B) were quantitated and plotted as the ratio of Bax/Bcl-2. (d) Apoptotic cells induced by the conditioned medium from LPS-stimulated and SCH-treated BV2 cells. Detection of apoptotic cells by Tunel method. Scale bar = 200 μm. Results were expressed as mean ± SD (n = 3). **p < 0.001 versus control; ##p < 0.01 versus LPS (1 μg/ml) group. Full-length blots are presented in Supplementary Fig. S2.

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