Figure 6 | Scientific Reports

Figure 6

From: Shikonin induces mitochondria-mediated apoptosis and enhances chemotherapeutic sensitivity of gastric cancer through reactive oxygen species

Figure 6

SHK induces loss of MMP, release of mitochondrial Cytochrome C, the caspase cascade and nuclear translocation of AIF and Endo G through ROS.

(a) MMP was detected by fluorescence microscopy (40×, Scale bars: 100 μm) and flow cytometry using JC-1 probes 12 h after SHK treatment. (b) NAC (1 mM) and GSH (1 mM) abolished the loss of MMP 12 h after SHK treatment (10 μM). (c) Western blots indicated that NAC (1 mM) and GSH (1 mM) abolished the release of mitochondrial Cytochrome C. VDAC1 was used as loading control of mitochondria. (d) NAC (1 mM) and GSH (1 mM) abolished the decrease in RIPK1 and the cleavage of caspase-3, -8, -9 and PARP. (e) NAC (1 mM) and GSH (1 mM) abolished the nuclear translocation of AIF and Endo G. PCNA was used as a nucleus loading control. Values are presented as the mean ± SD for three independent experiments. *P < 0.05, **P < 0.01.

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