Figure 3

Affinity of ASC for growth factors.

(A) Microscopic images of ASC scaffold and ASC scaffold incubated with FITC-bFGF was placed in under the fluorescence light and white light (×40, scale bar, 250 μm). (B) The bFGF-R of ASC scaffold by the immunohistochemical staining. Magnification was ×400. Scale bar, 50 μm. (C) CD spectra of bFGF solution and bFGF binding with ASC after 1 h of exposure to different temperature. The concentration of bFGF is 0.1 mg/ml. The concentration of ASC was 1 mg/ml. (Random sampling, n = 3).