Figure 2
From: Generation of kidney tubular organoids from human pluripotent stem cells
Specificity and cross-reactivity of anti-KSP antibody for human kidney.
(a) Relative expression levels of KSP was determined by real-time PCR for HEK293 cells, relative to the undifferentiated hESCs as a negative control. Transcript expression levels were normalized to GAPDH (n = 4–5). Values shown are the means ± SEM. P-values were determined by a Student’s t-test. *P < 0.05 (b) Western blot analysis of KSP expression in human aortic smooth muscle cells (HASMCs) and HEK293 cells (left). (right) Immunoblot with secondary antibody alone (2nd antibody alone). (c) Flow cytometric analysis of HEK293 cells labeled with our anti-KSP antibody. Blue line represents unstained cells and red represents cells stained with our anti-KSP antibody. HASMCs were used as the negative control. Flow cytometric analysis with our anti-KSP antibody showed positive cells in about 50% of HEK293 cells. (d) Immunohistochemistry of HEK293 cells using our anti-KSP antibody. (e–g) Human kidney tissue samples were labelled with antibodies against KSP alone (e) or in conjunction with AQP1 (f) or AQP2 (g). Nuclei were counterstained with DAPI. Scale bar; (d) 50 μm, (e–g) 20 μm.
