Figure 2
The influence of the PD-1/PD-L pathway on M.tb-specific IFN-γ secretion.
(A1) IFN-γ-secreting CD4+ T cells increased in both PBMCs and PFMCs of ATB patients stimulated with PPD (P = 0.0161 and P = 0.0087). Those in PFMCs were higher than those in PBMCs (P = 0.0411). (A2) The correlation between PD-1 expression and IFN-γ secretion of CD4+ T cells when stimulated with PPD was significant (r = 0.58; P = 0.011). (A3) Expression of PD-1 in IFN-γ-secreting CD4+ T cells stimulated with PPD in PBMCs and PFMCs of ATB group was both higher than that in PBMCs of the HC group (P = 0.0489 and P = 0.0201, respectively). (B1) The frequency of IFN-γ-secreting CD8+ T cells in PBMCs and PFMCs of ATB patients stimulated with PPD was similar. (B2) The correlation between PD-1 expression and IFN-γ secretion of CD8+ T cells when stimulated with PPD was significant (r = 0.56, P = 0.015). (B3) The expression of PD-1 in IFN-γ-secreting CD8+ T cells stimulated with PPD in PBMCs and PFMCs of the ATB group was similar to that in PBMCs of the HC group. (C) Representative CD4-gated histograms of PPD-specific IFN-γ secretion blocked with PD-1/PD-L pathway Abs. (D) After blocking of the PD-1/PD-L pathway with anti-PD-1 and/or anti-PD-L1/PD-L2 Abs, the IFN-γ secretion of CD4+ T cells was not significantly different from each other.
