Figure 2
From: Antimicrobial growth promoters modulate host responses in mice with a defined intestinal microbiota
Densities of Citrobacter rodentium associated with mice.
Animals were inoculated with C. rodentium (CR+) or PBS (CR−) that received feed without AGPs (CNRL), 5.5 mg of chlortetracycline/kg of feed (CTC), or 22 mg tylosin phosphate/kg feed (TYL). (A) Densities of live C. rodentium (log CFU/g) in feces as determined by dilution plating. (B) Densities of C. rodentium (log copies/g) in colonic ingesta at 7, 14, and 21 days p.i. as determined by quantitative PCR. Vertical lines associated with markers and histogram bars the SEM (n = 3–4). (C) Visualization of C. rodentium in colon tissues at peak infection (i.e. 7 days p.i.); cell nuclei are stained with DAPI (blue), total bacteria are stained with Alexa-488 (green) and γ-proteobacteria (C. rodentium) are stained with Alexa-594 (red). Scale bar = 50 μm (20X images) and 100 μm (40X images).
