Figure 2
From: Risk assessment of recent Egyptian H5N1 influenza viruses
Receptor-binding properties of Egyptian H5N1 and control viruses.
(A) Binding of viruses to sialylglycopolymers containing either α2,3-linked (blue) or α2,6-linked (red) sialic acids in solid-phase binding assays. Shown are the Dakahlia, Giza, and Cairo viruses, and control viruses expressing the human A/Kawasaki/173/2001 (K173; H1N1) or avian A/Vietnam/1203/2004 (VN1203; H5N1) HA and NA proteins in the background of A/Puerto Rico/8/34 (H1N1) virus. (B) Binding of viruses to human respiratory tissues. The viruses described above were labeled with FITC and incubated with human tissue sections. Tissue sections were subsequently incubated with HRP-conjugated, anti-FITC antibodies, and then with a chromogen substrate to detect virus binding. Dark red stain indicates virus binding.
