Figure 5
From: Regulation of human cardiac potassium channels by full-length KCNE3 and KCNE4
Effects of KCNE3L and hKCNE4L on human Kv4.3 current magnitude with/without KChIP2.
(A) Exemplar current traces recorded from Xenopus oocytes expressing human Kv4.3 alone (n = 14) or with human KCNE3L (E3L; n = 13), KCNE4L (E4L; n = 13), KCNE3S (E3S; n = 11), or KCNE4S (E4S; n = 9) (5 ng cRNA per oocyte of each subunit injected) Insets: left, scale bars; right, Voltage clamp protocol. Zero current level indicated by dashed line. (B) Mean ± SEM peak current/voltage relationship for currents as in panel A; n values as in panel A. See main text for statistics. (C) Exemplar current traces recorded from Xenopus oocytes expressing human Kv4.3 + KChIP2 alone (n = 12) or with human KCNE3L (E3L; n = 12) or KCNE4L (E4L; n = 11) (5 ng cRNA per oocyte of each subunit injected). Inset: scale bars. Zero current level indicated by dashed line. (D) Mean ± SEM peak current/voltage relationship for currents as in panel C; n values as in panel C. See main text for statistics. Inset, Kv4.3 current augmentation at 24 h and 48 h expression when 1 ng Kv4.3 cRNA was co-injected with 1 ng KChIP2 cRNA (n = 10–13). **p < 0.01; ***p < 0.001.
