Figure 4 | Scientific Reports

Figure 4

From: Intravenous hMSCs Ameliorate Acute Pancreatitis in Mice via Secretion of Tumor Necrosis Factor-α Stimulated Gene/Protein 6

Figure 4

Effects of hMSCs, scr siRNA hMSCs, TSG-6 siRNA hMSCs and rhTSG-6 on the production of inflammatory cytokines and chemokines.

(A) Immunohistochemical evaluation revealed the effects of each treatment on the expression level of TNF-α, IL-1β, IL-6, IL-4, IL-10, and MMP-9 in the pancreas of SAP mice. Original magnification: ×200. (B–G) The immunohistochemical scores of TNF-α, IL-1β, IL-6 and MMP-9 after operation were reduced, but IL-10 and IL-4 elevated, with i.v. administration of hMSCs, scr siRNA hMSCs and rhTSG-6 apart from TSG-6 siRNA hMSCs. Elisa assays for serum inflammatory cytokines (H) TNF-α, (I) IL-1β and (J) IL-6, (K) IFN-γ, and chemokines (L) FKN and (M) MCP-1. Each value represents the mean ± standard deviation (n = 8 or 12 per group). N.S., not significant, *P < 0.05 and **P < 0.01, compared with the SAP + PBS group.

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