Figure 2
From: Translocation of the thioesterase domain for the redesign of plipastatin synthetase
The linkers connecting the thioesterase (TE) domain with various thiolation (T) domains in new hybrid non-ribosomal peptide synthetases.
The 7ProT-C, 8GlnT-C, and 9TyrT-E linkers were connected to the TE domain, respectively (A–C). TELong, containing the native 10IleT-TE linker, was connecting to different T domains of the PPSD subunit (D–F). 7ProT-C: linker region between the T domain of module 7 and the condensation (C) domain of module 8. 8GlnT-C: linker region between the T domain of module 8 and the C domain of module 9. 9TyrT-E: linker region between the T and epimerization (E) domains in module 9.
