Table 2 Truncated lipopeptides produced by recombinant B. subtilis strains.

From: Translocation of the thioesterase domain for the redesign of plipastatin synthetase

Strain

Mass ions detected by LC-MS

Peptide sequence

Products

pB2-L

1435.8, 1449.8, 1463.8, 1477.8, 1491.8, 1505.8, 1519.8, 1533.8

(C14–21)β-OHFA-E-O-Cyclo(Y-T-E-A/V-P-Q-Y-I/V)

Plipastatin

LP4

1105.6, 1119.6, 1133.7, 1147.7

Linear (C16–19)β-OHFA-E-O-Y-T-E-V-P

Linear heptapeptides

1087.6, 1101.6, 1115.7, 1129.7

(C16–19)β-OHFA-E-O-cyclo(Y-T-E-V-P)

Cyclic heptapeptides

LP5

1233.7, 1247.7

Linear (C16–17)β-OHFA-E-O-Y-T-E-V-P-Q

Linear octapetides

1229.7, 1243.7, 1257.7

(C17–19)β-OHFA-E-O-cyclo(Y-T-E-V-P-Q)

Cyclic octapeptides

LP6

1382.7, 1396.8, 1410.8

Linear (C15–17)β-OHFA-E-O-Y-T-E-V-P-Q-Y

Linear nonapeptides

1219.7, 1233.7, 1247.7

Linear (C15–17)β-OHFA-E-O-Y-T-E-V-P-Q

Linear octapeptides

1215.7, 1229.7, 1243.7, 1257.7

(C16–19)β-OHFA-E-O-cyclo(Y-T-E-V-P-Q)

Cyclic octapeptides