Table 2 Truncated lipopeptides produced by recombinant B. subtilis strains.
From: Translocation of the thioesterase domain for the redesign of plipastatin synthetase
Strain | Mass ions detected by LC-MS | Peptide sequence | Products |
---|---|---|---|
pB2-L | 1435.8, 1449.8, 1463.8, 1477.8, 1491.8, 1505.8, 1519.8, 1533.8 | (C14–21)β-OHFA-E-O-Cyclo(Y-T-E-A/V-P-Q-Y-I/V) | Plipastatin |
LP4 | 1105.6, 1119.6, 1133.7, 1147.7 | Linear (C16–19)β-OHFA-E-O-Y-T-E-V-P | Linear heptapeptides |
1087.6, 1101.6, 1115.7, 1129.7 | (C16–19)β-OHFA-E-O-cyclo(Y-T-E-V-P) | Cyclic heptapeptides | |
LP5 | 1233.7, 1247.7 | Linear (C16–17)β-OHFA-E-O-Y-T-E-V-P-Q | Linear octapetides |
1229.7, 1243.7, 1257.7 | (C17–19)β-OHFA-E-O-cyclo(Y-T-E-V-P-Q) | Cyclic octapeptides | |
LP6 | 1382.7, 1396.8, 1410.8 | Linear (C15–17)β-OHFA-E-O-Y-T-E-V-P-Q-Y | Linear nonapeptides |
1219.7, 1233.7, 1247.7 | Linear (C15–17)β-OHFA-E-O-Y-T-E-V-P-Q | Linear octapeptides | |
1215.7, 1229.7, 1243.7, 1257.7 | (C16–19)β-OHFA-E-O-cyclo(Y-T-E-V-P-Q) | Cyclic octapeptides |