Figure 2
From: Production of Phloroglucinol, a Platform Chemical, in Arabidopsis using a Bacterial Gene
Expression analysis and quantification of PhlD transcript in transgenic lines.
Total RNA was isolated from wild-type (WT) and transgenic lines and used for RT-PCR (left panel, a,b) and qRT-PCR (right panel, c,d). Wild-type and selection marker Basta resistance gene (BAR) were used as negative and positive controls for the transgene, respectively. Primers specific to the chloroplast transit sequence (TP) were used to confirm the presence of chloroplast targeting signal sequence. Ubiquitin (UBQ5) primers were used as an internal control for normalization. Right, (c) and (d) show quantification of PhlD transcripts in transgenic lines as relative expression. The chloroplastic 19–2 transgenic line showed the highest expression. At 40 cycles no amplification product was detected in wild-type (WT) by qRT-PCR. Gels shown are cropped. All un-cropped gels are presented as “Un-cropped Fig. 2” in “Supplementary Figures and Tables file”.
