Figure 5
From: Production of Phloroglucinol, a Platform Chemical, in Arabidopsis using a Bacterial Gene
Validation of phlorin as the novel PG conjugate.
(a,b) Phlorin extract from orange peel (a) and the transgenic plant (b) extracts were treated with β-glycosidase enzyme mix (rapidase) and resolved in GC-MS before and after digestion showing that the phlorin peak disappears and the PG peak accumulates after digestion. (c) Arabidopsis leaf discs were incubated with PG in the presence or absence of D-glucose, extracted and analyzed by GC-MS. Orange peel extract was used as a control to indicate phlorin formation.
