Table 1 GC-MS quantification of free PG, PG released from enzymatic digestion of phlorin and total PG (mg/g) using PG standard.

From: Production of Phloroglucinol, a Platform Chemical, in Arabidopsis using a Bacterial Gene

Lines

PG (mg/g lyophilized tissue)

Free PG

Conjugated PG (phlorin)

Total PG

19–2 (chloroplastic)

5.7 (15.2%)

31.76 (84.8%)

37.46

23–2 (cytosolic)

0.28 (23.5%)

0.91 (76.5%)

1.19

  1. The unconjugated PG was quantified as described in the methods section. In addition, an equal amount of the extract was also digested with rapidase enzyme and the total PG was quantified. The amount of conjugated PG was calculated by subtracting the free form from the total.
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