Table 1 Duplication analysis of 726 bp fragment within ZRS region.

From: An increased duplication of ZRS region that caused more than one supernumerary digits preaxial polydactyly in a large Chinese family

Sample

CT (Mean)

CT (Std Dev)

ΔCT (Mean)

ΔCT (Std Err)

ΔΔCT

RQ

RQ (Min)

RQ (Max)

V7*

36.4793

1.1429

17.2882

0.6654

0.0000

1.0000

0.2779

3.5989

V8

37.2986

1.0209

16.8888

0.6036

−2.1623

0.9675

0.2573

3.3686

V6

32.5860

0.3316

15.2731

0.3931

−2.0151

4.0420

1.8971

8.6120

IV6

32.9437

0.9258

14.3565

0.6409

−2.9317

7.6299

2.2224

26.1942

IV9

32.7875

0.3269

15.4066

0.2396

−1.8816

3.6849

2.3238

5.8432

III3

33.0184

0.1990

15.8218

0.1169

−1.4664

2.7633

2.2065

3.4605

III6

32.9968

0.2862

15.5906

0.1712

−1.6976

3.2436

2.3329

4.5098

  1. RQ denotes relative quantity and was used here as the equivalent to relative copy number (RCN).
  2. *The unaffected family member acted as referential calibrator.
Back to article page