Table 2 Primers used in PCR mapping of the genetic context surrounding the mcr-1 gene in the Salmonella isolates from animals in China.
From: Clonal spread of mcr-1 in PMQR-carrying ST34 Salmonella isolates from animals in China
PCR Primera | Primer sequence (5′→3′) | Target DNA sequence | Position | PCR amplification in this studyb | Reference sequence | ||||
|---|---|---|---|---|---|---|---|---|---|
S01 | GDS78 | GDS79 | GDS82 | GDS141 | |||||
ISApl1-F | AGAATTTTACTTCCCCGAGCC | ISApl1 | 21995..22015 | + | − | − | − | − | pHNSHP45 (KP347127) |
M-1R | CGCGCCCATGATTAATAGCAA | mcr-1 | 22656..22676 | ||||||
nikB-F | GATTATTTTACGCCCGGAGCA | nikB | 19093..19113 | ND | + | + | + | + | pHNSHP45 (KP347127) |
M-2R | AGCAAGGCATTGTCATAAGCA | mcr-1 | 23699..23719 | ||||||
I-F | CAAAGACGCGGTACAAGCAAC | mcr-1 | 23081..23101 | ND | + | + | + | + | pHNSHP45 (KP347127) |
I-R | AAGACTCGTCCAACATATGGC | orf076 | 24907..24927 | ||||||
H-1F | AACGCTTCGAAATAACGGAT | orf00200 | 151220..151239 | − | ND | ND | ND | ND | pHNSHP45-2 (KU341381) |
H-1R | GTTGTATTTATTACCGATGGC | terF | 155812..155832 | ||||||
H-2F | ACCATGGACGTGAAAATGCT | — | 50090..50109 | + | ND | ND | ND | ND | pMR0516mcr (KX276657) |
H-2R | AGATTAGAGAGTGCCCCTCCC | — | 45927..45947 | ||||||
