Figure 4 | Scientific Reports

Figure 4

From: FOXA1, GATA3 and PPARɣ Cooperate to Drive Luminal Subtype in Bladder Cancer: A Molecular Analysis of Established Human Cell Lines

Figure 4

Candidate gene analysis suggests GATA3 and FOXA1 cooperate with PPARɣ activation to regulate molecular subtype-specific gene expression in bladder cancer cells.

(A) Western blotting analysis of a panel of human bladder cancer cell lines for expression of FOXA1 and PPARɣ. Cells are arranged by molecular subtype based on analysis presented in Fig. 1. (B) Q-RT-PCR analysis of human bladder cancer cell lines for GATA3. (C–F) Q-RT-PCR analysis for the luminal bladder cancer markers (C) FGFR3 and (D) KRT20, as well as the basal bladder cancer markers (E) EGFR and (F) KRT6 following individual overexpression of empty vector (CMV), FOXA1, GATA3, or FOXA1 and GATA3 in the presence of vehicle control (DMSO) or 1 micromolar rosiglitazone (TZD). See materials and methods for detailed description of experimental approach.

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