Figure 2
Immunoblot detection of proteins in HDL isolates.
HDL was isolated from 3 individual healthy donors by (A) two-step density gradient ultracentrifugation or (B) dextrane-sulfate precipitation as described in methods. HDL was probed for proteins which were according to mass spectrometry results (Table 1) exclusively associated either with the HDL2/3 fraction (apoA-I, apoE, apoC-I and SAA) or with the pre-β HDL containing fraction (apoA-IV, HSA and α-1-AT) (Table 1). HDL was separated by native gel electrophoresis, transferred to PDVF membranes and probed using specific antibodies as described in methods. Total protein was visualized with Panceau Red staining. α-1-AT, α-1-antitrypsin; apo, apoprotein; HSA, human serum albumin.
