Figure 4
From: Hypothalamic activation is essential for endotoxemia-induced acute muscle wasting
Hypothalamic neuropeptide POMC expression in saline or LPS treated rats after ARC injection of lentiviruses containing shRNA against POMC or control shRNA.
Two weeks after ARC viruses injection, rats were i.p. injected saline or LPS. Twenty four hours later, brain tissue were harvested for immunostaining as described in method. Data show POMC immunostaining (green) across the hypothalamic ARC of rats in experiment 2. DAPI staining (blue) reveals the nucleus of all cells in the sections (A). Bar = 50 μm. A subset of rats were used for the detection of POMC mRNA expression by real-time PCR method (B). GAPDH was used as control. Data are represented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.
