Figure 6
From: Hypothalamic activation is essential for endotoxemia-induced acute muscle wasting
Knocking down hypothalamic POMC expression can effectively attenuate endotoxemia-induced muscle wasting.
Twenty-four hours after treatment in experiment 2, rats were euthanized and EDL and gastrocnemius were harvested for measurement. Food intake (A), BW increase (B), EDL weight (C) and EDL weight normalized to initial BW (D) were measured. Atrophy gene (MuRF1, MAFbx and FOXO1) expression in rat gastrocnemius muscle were measured by real-time PCR (E). Reported values are relative to GAPDH. Net 3-MH and tyrosine release of EDL reflecting total protein breakdown were determined by HPLC (F and G). Gastrocnemius were immunostained for laminin (green) to delineate fiber area (H) and frequency distribution was calculated (I). Ultrastructure of myocyte and mitochondria under TEM (magnification*20000) (J). Data are represented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.
