Figure 1
From: NADPH oxidase 4 deficiency increases tubular cell death during acute ischemic reperfusion injury
NOX4 expression is upregulated after ischemia reperfusion injury (IRI).
(A) Representative images of cortico-medullary junction Haematoxylin Eosin staining depicting tubular injury are shown for Sham and 22 minutes ischemia reperfusion (IR) operated animals 24 hours after reperfusion. Scale bar 100 μm upper and 50 μm lower panels. (B) Serum creatinine measurement performed on sham and IR animals (Sham, n = 7; IR, n = 6) at 24 hours after IR. Results are expressed as absolute creatinine values in umol/l ± SEM, ns p > 0.05, *p < 0.05. (C) Representative images of NOX4 immunostaining performed in Sham (Sham, up) and IRI (IRI, down) kidneys from WT mice in the cortex and the cortico-medullar (CM) jonction. (D–F) NOX4 Western blot analysis performed on kidney cortex protein samples from sham and IR operated animals for the high (H) and low (L) molecular band. Representative picture and Western blot densitometric quantification are shown (Sham, n = 6; IR n = 5). Results are expressed as the mean of individual densitometric values over the mean densitometric value obtained in sham operated animals ± SE, ns p > 0.05 and *p < 0.05.
