Figure 4
From: NADPH oxidase 4 deficiency increases tubular cell death during acute ischemic reperfusion injury
NRF2 and NRF2 target genes expressions are downregulated in NOX4 KO mice under IRI.
(A,B) Western blot analysis of NRF2 performed on kidney cortex from NOX4 and WT mice after IR. Representative picture and densitometric quantification (WT, n = 3; NOX4 KO, n = 5) of Western blots are shown. Results are expressed as the mean of individual densitometric values of the mean of densitometric value obtained in WT animals ± SEM, ns p > 0.05, *p < 0.05. (C–E) Real time PCR analysis of GSTα2 (C), GCLC (D) and NQO1 (E) mRNA expressions performed in NOX4 KO and WT animals (WT, n = 10; NOX4, n = 9). Results are expressed as relative expression compared to WTs ± SEM, ns p > 0.05, *p < 0.05.
